The Secret Truth on Admission Essay Samples for Graduate School Uncovered

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Activated Carbon Cadmium

Sample details Pages: 17 Words: 5008 Downloads: 7 Date added: 2017/06/26 Category Statistics Essay Did you like this example? Introduction Environmental pollution by metals is a serious threat today due to increased industrial revolution and has become extensive. The current worldwide mine production of various heavy metals such as Cu, Cd, Pb, and Hg is very high (Pinto et al., 2004). These heavy metal sources include a number of diverse anthropogenic sources (textile, tannery and other industrial effluents, industrial solid wastes, urban runoff, sewage treatment plants, boating activities, agricultural fungicide runoff, domestic garbage dumps, and also by mining operations). Don’t waste time! Our writers will create an original "Activated Carbon Cadmium | Sciences Dissertations" essay for you Create order Metal toxicity and its effect on the growth and metabolism in higher plants is a subject that has a wide economical and ecological interest and also have been widely reviewed and gaining importance on several occasions over the last few decades (Brown and Jones, 1975; Foy et al., 1978; Ernst et al., 1992; Das et al., 1997; SanitÃÆ'Â ¡ di Toppi and Gabrielli, 1999; Hall, 2002; Clemens et al., 2002). Another interesting reason for this is out of the ninety naturally occurring elements fifty-three are heavy metals (Weast, 1984). Few metals are found to be essential for growth of plants, while few are found to be toxic for the growth of plants. Some of the heavy metals dont interact with the plant metabolism directly but decreases the level of soil microbes, affecting the growth of the plants (Niess, 1999). However, the accumulation in the soil has become a worldwide problem leading to the loss of agricultural productivity (Hasan, 2007). Among the heavy metals Cd is of special concern because of its potential toxicological profile even at lower concentration (Das Samantray, 1997). Cadmium is a toxic trace pollutant for humans, animals and plants, which enter the environment from various industrial processes, phosphate fertilizers and other anthropogenic activities, which then enters the food chain (Wagner, 1993). It has been estimated that more than 45% of all cadmium is used in electroplating, and that of 10% of total industrial cadmium use ends up in wastewater streams. Interests in the ecological effects of heavy metals has been growing recently, and assessing the phytotoxicity of cadmium is one of the most important criteria being used to avoid environmental risks, before these effluents can be recycled back for agricultural irrigation after some remediation or treatment processes. So it is inevitable that we should cope with the environmental damages induced by increased concentration of cadmium. The removal of cadmium (II) from wastewater stream therefore is of great importance as it can directly contaminate the ground water, aquatic organisms, agricultural lands and also the productivity. The global threat of reduction of uncontaminated ground water is also increasing exponentially with the scientific, industrial and technological developments (Tchobanoglous et al., 1991). Although various other techniques such as precipitation are most commonly used, to clean up the Cd contaminated water, the drawbacks of using those techniques, increases the need for an alternative technique which is more effective, reliable and also economically viable (USEPA, 1984). Cadmium being a highly toxic metal pollutant of soil inhibits root and shoot growth and yield production, affects nutrient uptake and homeostasis, and is frequently accumulated by agriculturally important crops including soybean with a significant potential to impair animal and human health (Sanita di toppi and Gabrielli, 1999). In plants such as pea, a number of toxic effects of Cd on metabolism has been reported, such as decreased uptake of nutrient elements (Sandalio et al., 2001), inhibition of various enzyme activities (Obata et al., 1996) and induction of oxidative stress (Lozano-Rodriet al., 1997, Romero-Puertas et al., 1999, Sandalio et al., 2001) including alterations in enzymes of antioxidant defense system (Dalurzo et al., 1997, Sandalio et al., 2001). However only little is known about the biochemical mechanisms underlying the intraspecific variation in the sensitivity versus tolerance to Cd in plants (Ashraf et al., 2005). Understanding the connections between a plants initial responses and the downstream events that constitute successful adjustment to its altered environment is one of the next grand challenges of plant biology (Ruth Grene, 2002). One of the common characteristics of these heavy metals such as cadmium is the induction of oxidative stress generating active oxygen species (AOS). Plants have evolved various protective defense mechanisms to eliminate or reduce these AOS species. One of them is the enzymatic or non-enzymatic antioxidant system, including peroxidase, Super oxide dismutase, catalase, glutathione peroxidase etc. Each of these parameters has physiological function under non-stressed conditions, but their activity or quantity is increased under oxidative stress (Sara Erdei, 2002). This study becomes more important because legume crops are less tolerant to Cd toxicity than cereals, grasses and frequently encounter strong inhibition of biomass production even at the less than micro molar range of cadmium (Inouhe et al., 1994). No much of the studies have been reported in the effect of cadmium in reproductive stage of soybean plant. Especially studies on the response of plants can very much help in increasing productivity, improving biomass, and reducing the green house gases, and also in the identification of Cd tolerant plants. Research studies on Cd effect on the economically important soybean crop may also be helpful to meet the increasing demand for soybean in India. The increasing global demand for soybean, oil and other products, rise in price at the international level particularly after the usage of soybean for biodiesel production, made us to select soybean as the plant of our research interest in this study. With the increasing scarcity for water and increasing pollution of ground water the irrigation of the legumes has become a big problem, in addition to the contaminated and less fertile soil for the farmers. Hence it is very essential to derive mechanisms to clean up technologies to remove the contaminants form wastewater and reuse of it for irrigation of legumes. Activated carbon absorption has been used successfully for the advanced treatment of municipal and industrial wastewater. Adsorption occurs when molecules adhere to the internal walls of pores in carbon particles produced by thermal or acid chemical activation (US EPA, 2000). Production of activated carbon from an easily available agro waste makes this technique more apt to adapt in a large-scale level. Also the reduction in the production cost by modification of already available techniques is very important and makes this process economically very feasible. Hence in our study we have selected the agricultural waste, shell from Anacardium occidentale L., a member of the Anacardiaceaefamily, of which the cashew is by far the most important economically. Anacardium occidentale L., is cultivated commercially in 32 countries on over 7.5 million acres. Cashew is now the #1 tree nut crop in the world, since its production surpassed that of almond in 2003. Average yields worldwide are about 600 lbs/acre. And India ranks second in its production contributing to 25% of the total production worldwide. Anacardium occidentale L produce 2 additional products of commercial value from their fruit: cashew apples and cashew nut shell liquid. After the CNSL is taken, the left out biological waste shell is thus considered for the production of low cost activated carbon in our study, which is available in higher quantity at a very cheaper price. Hence this study may be increasing one more commercially important product from the waste of Anacardium occidentale L. Objectives of The Study The major objectives of this research project are Use of the left out agro waste shell of Anacardium occidentale L., for the production of low cost activated carbon, in turn increasing one more commercially important product from the waste of Anacardium occidentale L., this also may be helping to improve the production of activated carbon in India to meet the increasing global need in the activated carbon industry. Development of an activated carbon process as the cleaning technology for removing heavy metal cadmium, from the Cd contaminated water Reuse of the treated water for growing economically important legume soybean and studying the response of soybean grown with the cadmium contaminated water before and after treatment. Specific Aims The specific aims of this study are as follows Study of the effect of Cd on the growth, stress and antioxidant metabolism of Glycine max (L.) Merr. Plants by petriplate and pot culture studies. Production and characterization of low cost activated carbon from the agro waste nut shell of Anacardium occidentale L. Batch mode experiments for the optimizing the conditions for maximum removal of Cd by the activated carbon from the biological waste nut shell of Anacardium occidentale L. Application of the treated water for irrigation and study of effect of Cd contaminated water after treatment on the growth, stress and antioxidant metabolism of Glycine max (L.) Merr. Plants by petriplate and pot culture studies. Evaluation of the effect of treated water on the growth on the growth of soybean over the treated water. A study on uptake and localization of cadmium by various parts of soybean Experimental Design and Salient Findings The experimental protocols are deduced and the work is designed in order to fulfill the objectives of the study and was carried out in eight phases, following standard protocols wherever necessary. PHASE-I: Collection of Seeds, Soil and Biological Waste Sample A. COLLECTION OF SEEDS, SOIL AND AGRO WASTE: Co-2 variety soybean [Glycine max (L.) Merr] seeds were collected from Millets and New Area Farm, Centre for Plant Breeding and Genetics, Tamilnadu Agricultural University, Coimbatore, and used for the study. The biological waste of shell of Anacardium occidentale L.was collected and the activated carbon is produced by the acid activation process. B. PREPARATION OF SYNTHETIC EFFLUENTS: The synthetic effluents with concentrations varying from 5, 10, 15, 20 and 25 ppm concentration of cadmium were prepared by using cadmium chloride, by calculating the level of cadmium in CdCl2 and used for growing the plants during germination, pot culture studies and for Cd adsorption studies. Distilled water is used as the control for all the experiments. C. COLLECTION OF THE SOIL AND SOIL STUDY Red soil was collected from the agricultural fields of Coimbatore, the composition is checked, and used throughout the study. The soil parameters checked includes, Moisture (%), pH, Electrical conductivity, Total nitrogen, Total phosphorus, Total potassium, Organic carbon, C / N ratio, Available Nitrogen, Available Phosphorus, Available potassium, Exchangeable calcium, Exchangeable Magnesium, Exchangeable sodium, Calcium, Magnesium, Sulphate, Chloride, Cadmium. Findings: The above parameters were checked to ensure that the soil is suitable for the growth of soybean. The Cd level was determined using Atomic Absorption Spectroscopy and found to be below non detectable limit and hence used for the study. PHASE-II Effect of Cadmium On Germination of Soybean (Petriplate Study 10 Days) The plants were grown in six groups as follows, and five plants were selected for checking germination parameters. GROUP-I NORMAL WATER TREATED SOYBEAN GROUP-II 5 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-III 10 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-IV 15 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-V 20 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-VI 25 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN Parameters Checked: The parameters checked on 10th day includes Germination percentage, Root length, Shoot length, Length of whole plant, Fresh mass of whole plant, Dry mass of whole plant, Dry mass / fresh mass ratio, Relative seed germination, Root length, Shoot length percentage, Germination index, Seed vigor index, Tolerance index, Germination rate Percentage. Findings of Germination Study Germination of soybean., seedlings was drastically affected by the increasing concentration of cadmium in the effluents. A significant decrease in germination percentage, root length, shoot length, fresh mass, dry mass of whole plant, relative seed germination, root length, shoot length percentage, germination index, seed vigor index, tolerance index and germination rate percentage was observed. Also a significant increase in dry mass / fresh mass ratio was observed. With these preliminary results obtained the work was extended to red soil study using pot culture. PHASE -III Pot Culture Studies EFFECT OF CADMIUM ON SOYBEAN OBSERVED ON 20TH DAY AND 40TH DAY: The plants were grown in six groups as follows, and five plants were selected for checking all physical parameters and all biochemical estimations were made on three plants selected as replications. GROUP-I NORMAL WATER TREATED SOYBEAN GROUP-II 5 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-III 10 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-IV 15 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-V 20 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-VI 25 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN III.A. GROWTH PARAMETERS CHECKED ON 20TH DAY The parameters checked on 20th day includes Root length, Shoot length, Plant height, Relative root length percentage, Relative shoot length percentage, Tolerance index, No of leaves, trifoliate, fresh weight, dry weight, Dry mass/fresh mass ratio, Leaf mass ratio, Stem mass ratio, Root mass ratio, Total dry matter yield under cadmium stress, Percentage weight gain, Seed vigor index, Leaf area measurements, Leaf area index, Leaf weight fraction, Specific leaf area, Leaf area ratio. FINDINGS: In the observations made with five plants in each group on 20th day, the following results were observed. The growth parameters such as root length, shoot length, plant height, relative root length percentage, relative shoot length percentage, tolerance index, no of leaves, trifoliates, fresh weight, dry weight were found to be decreased significantly with increasing concentrations of cadmium level in the effluents when compared to control. Also dry mass/fresh mass ratio, stem mass ratio, root mass ratio were found to be increased, while the other parameters such as leaf mass ratio, total dry matter yield under cadmium stress, percentage weight gain, seed vigor index, leaf area measurements, leaf area index, leaf weight fraction, specific leaf area, leaf area ratio were also found to be decreasing when compared to control group plants, with increasing levels of cadmium in the effluents. III.B. GROWTH PARAMETERS CHECKED ON 40TH DAY The parameters checked on 20th day includes Root length, Shoot length, Total plant height, Relative root length percentage, relative shoot length percentage, tolerance index, Number of leaves, trifoliate, nodules per plant, fresh weight, dry weight of individual organs, and whole plant, Dry mass/fresh mass ratio of individual organs, Leaf mass ratio, Stem mass ratio, Root mass ratio, Total dry matter yield under cadmium stress, Percentage weight gain, Seed vigor index, Leaf area measurements, Leaf area index, Leaf weight fraction, Specific leaf area, Leaf area ratio, Absolute growth rate, Crop growth rate, Unit leaf rate, Net assimilation rate, Relative growth rate, Leaf area duration FINDINGS: In the observations made with five plants in each group on 40th day, the following results were observed. The growth parameters such as root length, shoot length, plant height, relative root length percentage, relative shoot length percentage, tolerance index, number of leaves, trifoliates, nodules per plant, fresh weight, dry weight of individual organs, and whole plant, leaf mass ratio, total dry matter yield under cadmium stress, percentage weight gain, seed vigor index, leaf area measurements, leaf area index, leaf weight fraction, specific leaf area and leaf area ratio were found to be decreased significantly. The dry mass/ fresh mass ratio, stem mass ratio and root mass ratio were found to be increasing with increase in the concentration of cadmium. The growth parameters connecting the observations made on 20th and 40th day observations such as absolute growth rate, crop growth rate, unit leaf rate, net assimilation rate, relative growth rate, leaf area duration values showed a significant difference from the values of control group plants. III.C. BIOCHEMICAL PARAMETERS ESTIMATED ON 20TH DAY IN ROOT AND SHOOT TISSUES: The various biochemical estimations made includes, i. GROWTH PARAMETERS Chlorophyll a, b, total chlorophyll, Total carbohydrate, Starch, total Protein ii. ENZYMES: Amylase alpha, beta and total, Glucose-6-phosphate dehydrogenase iii. STRESS MARKERS Total free amino acids, Proline, TBARS iv. NON ENZYMATIC ANTIOXIDANTS Vitamin E, Vitamin C, Reduced Glutathione v. ENZYMATIC ANTIOXIDANTS Superoxide dismutase, Catalase, Peroxidase, Glutathione Reductase, Glutathione Peroxidase. FINDINGS: The levels of growth parameters such as Chlorophyll-a, b and total Chlorophyll were significantly found to be affected with the increase in the cadmium concentrations in the effluents. The levels of Total Carbohydrate, Starch and Total Protein were also estimated in root and leaf tissues and were found to be significantly decreased with increasing concentrations of cadmium. The decreasing levels of Amylase, Glucose-6-phosphate dehydrogenase were also observed with increasing concentrations of cadmium, when compared to control in both root and leaf tissues. The stress markers like Total Free Amino Acids, Proline and Thiobarbituricacid reactive substances (TBARS) were also found to be increased with increase in the concentrations of cadmium in both leaf and root tissues. The non enzymatic antioxidants such as Vitamin E, Ascorbic acid, and Reduced Glutathione were found to significantly varying with the varying concentrations of cadmium. The enzymatic antioxidants such as Catalase, Peroxidase, Glutathione Reductase, Glutathione peroxidase were found to be decreased in their levels with increasing concentration of cadmium. Superoxidedismutase was found to be increasing with increase in the Cd concentration. PHASE-IV PRODUCTION AND USE OF LOW COST ACTIVATED CARBON FOR REMOVAL OF CADMIUM FROM CONTAMINATED EFFLUENT ADSORPTION STUDIES. COLLECTION OF THE BIOLOGICAL WASTE. ACTIVATED CARBON PRODUCTION BY ACID ACTIVATION. PHYSICAL PROPERTIES OF ACTIVATED CARBON FTIR ANALYSIS OF ACTIVATED CARBON BEFORE ADSORPTION SEM ANALYSIS BEFORE ADSORPTION FINDINGS: The agricultural waste, shell from Anacardium Occidentale L., were collected, and the activated carbon is produced, the activated carbon of size less than 125 micron was selected and its physical parameters were determined, and is found to be suitable for removal of cadmium from waste water. Fourier Transform Infra Red spectroscopy and Scanning Electron Microscopy analysis were done for the prepared activated carbon. PHASE-V BATCH MODE ADSORPTION STUDIES Batch mode adsorption studies were done for the following parameters EFFECT OF EQUILIBRIUM TIME EFFECT OF pH EFFECT OF VARYING CARBON CONCENTRATION EFFECT OF VARYING CADMIUM CONCENTRATION EFFECT OF TEMPERATURE FINAL PERCENTAGE REMOVAL OF CADMIUM FROM VARIOUS CONC OF Cd SOLUTIONS. FTIR ANALYSIS OF ACTIVATED CARBON AFTER ADSORPTION SEM ANALYSIS AFTER ADSORPTION FINDINGS: The batch mode experiments were performed for adsorption studies and the optimum equilibrium time, optimum pH, effects of varying carbon concentration and varying carbon concentration and temperature were done and the carbon to cadmium ratio was determined. The final percentage of cadmium removed successfully after treatment with optimum concentrations was determined using Atomic Absorption Spectroscopy. The carbon collected after adsorption was analyzed using Fourier Transform Infra Red spectroscopy and Scanning Electron Microscopy. PHASE-VI EFFECT OF CADMIUM TREATED WATER ON GERMINATION OF SOYBEAN (PETRIPLATE STUDY 10 DAYS) The plants were grown in six groups as follows, and five plants were selected for checking germination parameters. GROUP-I NORMAL WATER TREATED SOYBEAN GROUP-II 5 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-III 10 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-IV 15 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-V 20 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-VI 25 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROWTH PARAMETERS CHECKED: The parameters checked on 10th day includes Germination percentage, Root length, Shoot length, Length of whole plant, Fresh mass of whole plant, Dry mass of whole plant, Dry mass / fresh mass ratio, Relative seed germination, Root length, Shoot length percentage, Germination index, Seed vigor index, Tolerance index, Germination rate percentage FINDINGS OF GERMINATION STUDY: Although the test groups showed a reduction in parameters when compared to control group, a highly significant improvement in the germination of soybean seedlings grown using with the increasing concentration of cadmium in the effluents after treatment. A significant improvement in germination percentage, root length, shoot length, fresh mass, dry mass of whole plant, relative seed germination, relative root length, shoot length percentage, germination index, seed vigor index, tolerance index and germination rate percentage were observed when compared to before treatment plants. Also a significant variation in dry mass / fresh mass ratio was observed. With these significant preliminary results obtained for treated effluents, the work was extended to red soil study using pot culture. PHASE- VII EFFECT OF CADMIUM ON METABOLISM AND ANTIOXIDANT STATUS OF SOYBEAN ON 20TH DAY AND 40TH DAY: The plants were grown in six groups as follows, and five plants were selected for checking all physical parameters and all biochemical estimations were made on three plants selected as replications. GROUP-I NORMAL WATER TREATED SOYBEAN GROUP-II 5 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-III 10 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-IV 15 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-V 20 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-VI 25 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN VII.A. GROWTH PARAMETERS MEASURED ON 20TH DAY The parameters checked on 20th day includes Root length, Shoot length, Plant height, Relative root length percentage, Relative shoot length percentage, Tolerance index, Number of leaves, Trifoliate, Fresh weight, Dry weight, Dry mass/fresh mass ratio, Leaf mass ratio, Stem mass ratio, Root mass ratio, Total dry matter yield under cadmium stress, Percentage weight gain, Seed vigor index, Leaf area measurements, Leaf area index, Leaf weight fraction, Specific leaf area, Leaf area ratio FINDINGS: The following observations were made with five plants in each group grown with treated effluents, on 20th day: The growth parameters such as root length, shoot length, plant height, relative root length percentage, relative shoot length percentage, tolerance index, number of leaves, trifoliates, fresh weight, dry weight were found to be decreased when compared to control. But a highly significant improvement was seen in all these parameters, when compared with the values of plants grown with effluents before treatment. Also dry mass/fresh mass ratio, stem mass ratio, root mass ratio were also found to be varied than the before treatment plants. The other growth parameters such as leaf mass ratio, total dry matter yield under cadmium stress, percentage weight gain, seed vigor index, leaf area measurements, leaf area index, leaf weight fraction , specific leaf area, leaf area ratio were also found to be improved when compared to before treatment plants, although it is decreasing when compared to control group plants. VII.B. GROWTH PARAMETERS MEASURED ON 40TH DAY The parameters checked on 20th day includes Root length, Shoot length, Plant height, Relative root length percentage, Relative shoot length percentage, Tolerance index, Number of leaves, trifoliate, nodules per plant, Fresh weight, Dry weight of individual organs and whole plant, Dry mass/fresh mass ratio of individual organs, Leaf mass ratio, Stem mass ratio, Root mass ratio, Total dry matter yield under cadmium stress, Percentage weight gain, Seed vigor index, Leaf area measurements, Leaf area index, Leaf weight fraction, Specific leaf area, Leaf area ratio , Absolute growth rate, Crop growth rate, Unit leaf rate, Net assimilation rate, Relative growth rate, Leaf area duration FINDINGS: In the observations made with five plants in each group on 40th day, the following results were observed for the plants grown after treatment of Cd containing waste water: The growth parameters such as root length, shoot length, plant height, relative root length percentage, relative shoot length percentage, tolerance index, no of leaves, trifoliates, nodules per plant, fresh weight, dry weight of individual organs, and whole plant, leaf mass ratio, total dry matter yield under cadmium stress, percentage weight gain, seed vigor index, leaf area measurements, leaf area index, leaf weight fraction, specific leaf area, leaf area ratio were found to be increased significantly when compared with the before treatment plants. The dry mass/ fresh mass ratio, stem mass ratio and root mass ratio were also found to be varying with concentration of cadmium after treatment. The growth parameters connecting the observations made on 20th and 40th day observations such as absolute growth rate, crop growth rate, unit leaf rate, net assimilation rate, relative growth rate, leaf area duration values showed a significant improved performance when compared to the plants gr own with the cadmium contaminated waste water before treatment. VII.C. BIOCHEMICAL PARAMETERS ESTIMATED ON 20TH DAY ON ROOT SHOOT TISSUES OF SOYBEAN: The various biochemical estimations made includes, i. GROWTH PARAMETERS Chlorophyll a, b, total chlorophyll, Total carbohydrate, Starch, Total Protein ii. ENZYMES: Amylase alpha, beta and total, Glucose-6-phosphate dehydrogenase iii. STRESS MARKERS Total free amino acids, Proline, TBARS iv. NON ENZYMATIC ANTIOXIDANTS Vitamin E, Vitamin C, Reduced Glutathione v. ENZYMATIC ANTIOXIDANTS Superoxidedismutase, Catalase, Peroxidase, Glutathione Reductase, Glutathione Peroxidase. FINDINGS: The levels of growth parameters such as Chlorophyll-a, b and Total Chlorophyll were significantly found to be improved with the increase in the cadmium concentrations in the effluents after treatment. The levels of Total Carbohydrate, Starch and Total Protein were also found to be increased significantly when compared to before treatment plants in both root and leaf tissues. Although a decreasing levels of Amylase, Glucose-6-phosphate dehydrogenase were observed with increasing concentrations of cadmium, when compared to control, a significant increase in their levels was observed when compared with the plants grown before treatment in both root and leaf tissues. The stress markers like Total Free Amino Acids, Proline and Thiobarbituric Acid Reactive Substances(TBARS) were also found to be decreased in both leaf and root tissues when compared with the plants grown using effluents before treatment. The non enzymatic antioxidants such as Vitamin E, Ascorbic acid and Reduced Glutathione were found to significantly varying with the varying concentrations of cadmium after treatment. The enzymatic antioxidants such as Catalase, Peroxidase, Glutathione Reductase, and Glutathione Peroxidase were found to be significantly increased in the plants grown after treatment. Also level of Superoxide dismutase was found to be reduced than the level found in plants grown using effluents before treatment. PHASE-VIII UPTAKE AND LOCALIZATION OF CADMIUM BY SOYBEAN PLANTS BEFORE TREATMENT The level of cadmium uptake was measured by atomic absorption spectrophotometer in the tissues of root, shoot, leaves, and pod of soybean grown using Cd contaminated water before treatment. AFTER TREATMENT The level of cadmium uptake was measured by atomic absorption spectrophotometer in the tissues of root, shoot, leaves, and pod of soybean grown using Cd contaminated water after treatment. FINDINGS: The uptake and localization of cadmium levels and correlated to the results obtained in biochemical and physical parameters. Conclusions The results obtained from the present study indicates The effect of Cd on the growth, stress and antioxidant metabolism of Glycine max (L.) Merr. Plants by petriplate and pot culture studies were studied and are found to be significant. The production of low cost activated carbon from agro waste of Anacardium occidentale L.., and the removal of Cd from the waste water by the low cost AC are significant. Use of the treated water for irrigation and its effect on the growth, stress and antioxidant metabolism of Glycine max (L.) Merr. Plants by petriplate and pot culture were studied. The results may be helpful in better understanding of the various growth, metabolic and defense response mechanisms of the Glycine max (L.) Merr. plants, grown with Cd contaminated water before and after treatment using activated carbon. The effect of treated water on the growth over the non-treated water was evaluated and the results show the efficiency of the treated Cd contaminated waste water for irrigation. The results also suggest that this method may be useful as a secondary treatment method in the water treatment plants involved in treatment of sewage wastewaters. References Ashraf Metwally, Vera I Safronova, Andrei A Belimov and Karl-Josef Dietz., Genotypic variation of the response to cadmium toxicity in Pisum sativum l., Journal of Experimental Botony, 56: 167-178, 2005. Brown JC, Jones WE., Heavy metal toxicity in plants 1. A crisis in embryo. Commun.Soil. Sci.Plant Anal., 6:421-438, 1975. Clemens S, Palmgreen MG, Kramer U., A long way ahead: Understanding and Engineering Plant Metal Accumulation. Trends Plant Sci., 7:309-315, 2002. Dalurzo HC, Sandalio LM, Gomez M, Del Rio LA, Cadmium Infiltration of Detatched Pea Leaves: Effect on its Activated Oxygen Metabolism. Phyton, 37: 59-64, 1997. Das P, Samantaray S, Rout GR, Studies on Cadmium Toxicity in Plants: A Review., Environ. Pollution 98: 29-36, 1997. Ernst WHO, Verkleij JAC, Schat H, Metal Tolerance in Plants., Acta Bot. Neerl. 41:229-248, 1992. Foy CD, Chaney RL, White MC.,The Physiology of Metal Toxicity in Plants., Ann. Rev. Plant Physiol. 29:11-566, 1978. Hall JL, Cellular Mechanisms for Heavy Metal Detoxification and Tolerance. J.Exp.Bot. 53:1-11, 2002. Hasan Syed Aiman, Barket Ali, Shamsul Hayat, Aqil Ahmed, Cadmium Induced Changes in the Growth and Carbonic Anhydrase Activity of Chickpea., Turk J Biol, 31: 137-140, 2007. Inouhe M, Ninomiya S, Tohoyama H, Joho M, Murayama T., Different Characteristics of Roots in the Cadmium Tolerance and Cd Binding Complex Formation between Mono- and Dicotyledonous Plants. Journal of plant research, 107: 201-207, 1994. Lozana-Rodriguez E, Hernandez L E, Bonany P, Carpena-Ruiz Ro. Distribution of Cd in Shoot and Root Tissues of Maize and Pea Plants: Physiological Disturbances., Journal of Exp Bot., 48: 123-128, 1997. Niess DH, Microbial heavy-metal resistance. Applied Microbiol. Biotech. 51:730-750, 1999. Obata H, Inouhe N, Umebayashi M., Effect of Cd on Plasma Membrane ATPase from Plant Roots Differing in Tolerance to Cd., Soil science and plant nutrition, 42: 361-366, 1996. Pinto AP, Mota AM, De Varennes A, Pinto FC., Influence of organic matter on the uptake of Cadmium, Zinc, Copper and Iron by Sorghum Plants., Sci.Tot. Environ., 326: 239-247, 2004. Romero-Puertas MC, Mc Carthy I , Sandalio LM, Palma JM, Corpas FJ, Gomez M, Del Rio LA., Cadmium Toxicity and Oxidative Metabolism of Pea Leaf Peroxisomes. Free Radical Research., 31: 25-31, 1999. Ruth Grene, Oxidative Stress and Accimilation Mechanisms in Plants., The Arabidopsis Book, American Society of Plant Biologists, 2002. Sandalio LM, Dalurzo HC, Gomez M, Romero-Puertas MC, Del Rio LA., Cadmium induced changes in the growth and oxidative metabolism of Pea plants., Journal of Experimental Botony, 52: 2115-2126, 2001. SanitÃÆ'Â ¡ di Toppi L, Gabbrielli R., Response to Cadmium in Higher Plants. Environ. Exp. Bot. 41:105-130, 1999. Sanita Di Toppi L, Gabrielli R., Response to Cadmium in Higher Plants., Environmental and experimental botany, 41: 105-130, 1999. Sara Erdei, Attila Hegedus, Gabor Hauptmann, Jozsef Szalai, Gabor Horvath., Heavy Metal Induced Physiological Changes in the Antioxidative Response System., Acta Biologica Szegediensis., 46(3-4): 89-90, 2002. Tchobanoglous, George and Burton, Fraklin L., Waste Water Engineering Treatment Disposal, Reuse., Metcalf and Eddy Inc., 3rd ed., 1991. USEPA, Granular Activated Carbon Systems Problems and Remedies, USEPA 800/490/9198, US EPA, Washington DC., 1984. Wagner G J., Accumulation of Cadmium in Crop Plants and its Consequences to Human Health. Adv Agron, 51: 173-212, 1993. US EPA, Waste Water Technology Fact Sheet, United States Environment Protection Agency, USEPA 832-f-00-017, US EPA, Washington DC., 2000. Weast RC, CRC Handbook of Chemistry and Physics. 64th Edn. CRC Press, Boca Raton, 1984.

The Role Of D Cycloserine ( Dcs ) On The Extinction Of...

In a study by Vengeliene, Kiefer, and Spanagel (2008), the role of d-cycloserine (DCS) on the extinction of conditioned alcohol-seeking behavior is investigated. In a dual-lever operant chamber, Wistar rats were trained using operant conditioning to press a lever to self-administer ethanol, while the opposite lever released water. Olfactory stimuli in the form of orange flavour extract and anise extract was added to aid the rats in discriminating between ethanol and water availability respectively. In addition to predictive olfactory stimuli, response-contingent cues were presented after each lever press. Through classical conditioning, ethanol is repeatedly paired with a conditioned auditory stimulus, similarly water self-administration results in the presentation of a light stimulus. The subjects were then divided into two groups, one group was injected with a vehicle and the other was injected with 5mg/kg DCS 60 minutes prior to each extinction session. During the twelve consecuti ve daily extinction sessions, both levers were extended with olfactory stimuli present and lever responses resulted in their associated cues, but no ethanol or water was released. One day after the last extinction session, the animals were primed with a dose of ethanol, and their ethanol responding was tested with its associated olfactory stimuli and response-contingent cue to study relapse-like behavior. This animal study found that the extinction of alcohol-seeking behavior, i.e. lever

Endurance Essay Example For Students

Endurance Essay Endurance plays a major role in many athletic activities. Without endurance training, many athletes would not be able to compete in their respective sports. Endurance training involves low resistance and high repetitions, but what exactly does this do to your body and how does it do it?For starters, endurance training increases the level of certain aerobic enzymes, which are needed for the breakdown of fuels to produce energy. Enzymes are proteins that speed up metabolic reactions; they release and transfer energy. Enzymes are influenced by many factors:1) Temperature- activity is increased when the muscles are warm, therefore warming up before the actual activity is very important2) Acidity- lower Ph levels reduces enzyme activity, leading to fatigue3) Fuel- enzymes work faster when they have more fuelThe faster the enzyme can breakdown your body’s fuel, the faster you will receive energy. Training also increases the number and size of the mitochondria. Mitochondria are cellular powerhouses that produce energy aerobically. All oxidative energy production takes place in the mitochondria. Oxygen is the key to endurance training, so the mitochondria are very important. Oxygen is needed to breakdown carbohydrates and fats, which provide energy. Dr. John Holloszy conducted an experiment to prove the importance of the mitochondria in endurance training. He had two groups of rats. One group was trained and eventually could exercise continually for four to eight hours. The untrained rats became tired after thirty minutes of exercise. Holloszy found a fifty to sixty percent increase in the mitochondrial protein and a twofold increase in oxygen consumption in the muscles of the trained rats. This also led to the conclusion that endurance-trained muscle fibers are better able to burn fat as a source of energy. Endurance training causes only limited gain in muscles. Muscles not in volved in training may actually lose strength. However, there may be an increase in myofibrils, but no significant increase in muscle fibers. Evidence has shown that fast-twitch fibers take on oxidative capabilities and assume the properties of slow-twitch fibers. Long distance runners have as much as eighty- percent slow-twitch fibers. Training also causes an increase in the ability of fast-twitch fibers to utilize oxygen. Endurance training also affects cardiac performance. The most obvious change that training brings about is a decrease in heart rate at both resting and at a fixed intensity. The stroke volume at all levels of physical activity is increased. The leads to a decrease in blood pressure. There is also an increase in cardiac output; cardiac output is the primary determinant of oxygen transport. Respiratory performance is affected by endurance training. It increases the endurance of chest muscles, which minimizes the loss of respiratory performance. Training also reduces the time it takes to reach a steady breathing rate once the exercise has begun. The ventilatory demands are decreased for four reasons:1) Increase in muscular efficiency2) Decrease in ventilatory drive3) Reduction in sensitivity of carotid chemorecptors4) Lesser production of lactateEndurance training can cause changes in your endocrine/hormonal system. Studies have shown the adrenal glands, which secrete epinephrine (adrenaline), enlarge with training. Epinephrine, cortisol, thyroxine, glucagon, and growth hormones are all secreted during exercise. Epinephrine and growth hormones are involved in the mobilization of fat. The need for insulin, which is usually secreted after a meal, is lowered because the muscles can take up sugar during exercise. Overall, training seems to fine tune the body’s secreti on and response to hormones. This leads to a more efficient use of hormones and energy. The previous talks about what endurance training does to your body. Now let’s look at what it does to you psychological. Endurance athletes have been found to have healthier mood state profiles. There are generally classified as having lower levels of tension, depression, anger, fatigue, and confusion. However, during periods of over-training, levels of anger, anxiety, and depression are increased. Overall though, endurance athletes are more mentally healthy than an average athlete is. .u92f8efc5bfa6f23e7475d779e96c3c6a , .u92f8efc5bfa6f23e7475d779e96c3c6a .postImageUrl , .u92f8efc5bfa6f23e7475d779e96c3c6a .centered-text-area { min-height: 80px; position: relative; } .u92f8efc5bfa6f23e7475d779e96c3c6a , .u92f8efc5bfa6f23e7475d779e96c3c6a:hover , .u92f8efc5bfa6f23e7475d779e96c3c6a:visited , .u92f8efc5bfa6f23e7475d779e96c3c6a:active { border:0!important; } .u92f8efc5bfa6f23e7475d779e96c3c6a .clearfix:after { content: ""; display: table; clear: both; } .u92f8efc5bfa6f23e7475d779e96c3c6a { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .u92f8efc5bfa6f23e7475d779e96c3c6a:active , .u92f8efc5bfa6f23e7475d779e96c3c6a:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .u92f8efc5bfa6f23e7475d779e96c3c6a .centered-text-area { width: 100%; position: relative ; } .u92f8efc5bfa6f23e7475d779e96c3c6a .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .u92f8efc5bfa6f23e7475d779e96c3c6a .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .u92f8efc5bfa6f23e7475d779e96c3c6a .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .u92f8efc5bfa6f23e7475d779e96c3c6a:hover .ctaButton { background-color: #34495E!important; } .u92f8efc5bfa6f23e7475d779e96c3c6a .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .u92f8efc5bfa6f23e7475d779e96c3c6a .u92f8efc5bfa6f23e7475d779e96c3c6a-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .u92f8efc5bfa6f23e7475d779e96c3c6a:after { content: ""; display: block; clear: both; } READ: Hamlet: The Dog will have his Day EssayEndurance is gained by persistency with a lighter load. This means doing maybe one set of 20-40 with ten pounds, instead of two sets of ten to twelve with twenty pounds. You progressively increase your workload. Consistency is very important. Endurance workouts are all week long, all year-round. Endurance training affects every part of your body, physically and emotionally. It makes you healthier and overall happier person. Endurance plays a major role in all of our athletic activities. Sports and Games